Electricity deregulation and the valuation of visibility loss in wilderness areas: A research note.

Visibility in most wilderness areas in the northeastern United States has declined substantially since the 1970s. As noted by Hill et al. (2000), despite the 1977 Clean Air Act and subsequent amendments, human induced smog conditions are becoming increasingly worse. Average visibility in class I airsheds, such as the Great Gulf Wilderness in New Hampshire’s White Mountains, is now about one-third of natural conditions. A particular concern is that deregulation of electricity production could result in further degradation because consumers may switch to lower cost fossil fuel generation (Harper 2000). To the extent that this system reduces electricity costs, it may also affect firm location decisions (Halstead and Deller 1997). Yet, little is known about the extent to which consumers are likely to make tradeoffs between electric bills and reduced visibility in nearby wilderness areas. This applied research uses a contingent valuation approach in an empirical case study of consumers’ tradeoffs between cheaper electric bills and reduced visibility in New Hampshire’s White Mountains. We also examine some of the problems associated with uncertainty with this type of analysis; that is, how confident respondents are in their answers to the valuation questions. Finally, policy implications of decreased visibility due to electricity deregulation are discussed

Global Implications of U.S. Biofuels Policies in an Integrated Partial and General Equilibrium Framework

With the increasing research interests in biofuels, global implications of biofuels production have been generally examined either in a partial equilibrium (PE) or general equilibrium (GE) frameworks. Though both of these approaches have unique strengths, they also suffer from many limitations due to complexity of addressing all the relevant aspects of biofuels. In this paper we have exploited the strengths of both PE and GE approaches for analyzing the economic and environmental implications of the U.S. policies on corn-ethanol and biodiesel production. In this study, we utilize the Forest and Agricultural Sector Optimization Model (FASOMGHG: Adams et al. 1996, 2005; Beach et al. 2009), a non-linear programming, PE model for the United States. We also use the GTAP-BIO model (Birur et al. 2008), a multi-region, multi-sector CGE model for global-scale assessment of biofuels policies. Following Britz and Hertel (2009), we link the GTAP-BIO model through a static, quadratic restricted revenue function obtained from perturbing crop prices from the FASOMGHG model. With this linkage we implement the U.S. Corn ethanol and biodiesel scenarios in the GTAP-BIO model and obtain the FASOMGHG-consistent, global land use changes. The resulting crop price changes from the GE model are fed back into the FASOMGHG model to obtain the disaggregated impacts in the U.S.Biofuels, Indirect land use change, Land use emissions, Partial Equilibrium, Computable General Equilibrium, Land Economics/Use, Resource /Energy Economics and Policy,

Long-range electron transfer in structurally engineered pentaammineruthenium (histidine-62) cytochrome c

In many biological processes, long-range electron transfer (ET) plays a key role. When the three-dimensional structures of proteins are accurately known, use of modified proteins and protein-protein complexes provides an experimental approach to study ET rates between two metal centers. For Ru(His)- modified proteins, the introduction of histidine residues at any desired surface location by site-directed mutagenesis opens the way for systematic investigations of ET pathways

Towards a membrane proteome in Drosophila: a method for the isolation of plasma membrane

<p>Abstract</p> <p>Background</p> <p>The plasma membrane (PM) is a compartment of significant interest because cell surface proteins influence the way in which a cell interacts with its neighbours and its extracellular environment. However, PM is hard to isolate because of its low abundance. Aqueous two-phase affinity purification (2PAP), based on PEG/Dextran two-phase fractionation and lectin affinity for PM-derived microsomes, is an emerging method for the isolation of high purity plasma membranes from several vertebrate sources. In contrast, PM isolation techniques in important invertebrate genetic model systems, such as <it>Drosophila melanogaster</it>, have relied upon enrichment by density gradient centrifugation. To facilitate genetic investigation of activities contributing to the content of the PM sub-proteome, we sought to adapt 2PAP to this invertebrate model to provide a robust PM isolation technique for <it>Drosophila</it>.</p> <p>Results</p> <p>We show that 2PAP alone does not completely remove contaminating endoplasmic reticulum and mitochondrial membrane. However, a novel combination of density gradient centrifugation plus 2PAP results in a robust PM preparation. To demonstrate the utility of this technique we isolated PM from fly heads and successfully identified 432 proteins using MudPIT, of which 37% are integral membrane proteins from all compartments. Of the 432 proteins, 22% have been previously assigned to the PM compartment, and a further 34% are currently unassigned to any compartment and represent candidates for assignment to the PM. The remainder have previous assignments to other compartments.</p> <p>Conclusion</p> <p>A combination of density gradient centrifugation and 2PAP results in a robust, high purity PM preparation from <it>Drosophila</it>, something neither technique can achieve on its own. This novel preparation should lay the groundwork for the proteomic investigation of the PM in different genetic backgrounds in <it>Drosophila</it>. Our results also identify two key steps in this procedure: The optimization of membrane partitioning in the PEG/Dextran mixture, and careful choice of the correct lectin for the affinity purification step in light of variations in bulk membrane lipid composition and glycosylation patterns respectively. This points the way for further adaptations into other systems.</p

Cell-Type Specific Changes in Glial Morphology and Glucocorticoid Expression During Stress and Aging in the Medial Prefrontal Cortex.

Repeated exposure to stressors is known to produce large-scale remodeling of neurons within the prefrontal cortex (PFC). Recent work suggests stress-related forms of structural plasticity can interact with aging to drive distinct patterns of pyramidal cell morphological changes. However, little is known about how other cellular components within PFC might be affected by these challenges. Here, we examined the effects of stress exposure and aging on medial prefrontal cortical glial subpopulations. Interestingly, we found no changes in glial morphology with stress exposure but a profound morphological change with aging. Furthermore, we found an upregulation of non-nuclear glucocorticoid receptors (GR) with aging, while nuclear levels remained largely unaffected. Both changes are selective for microglia, with no stress or aging effect found in astrocytes. Lastly, we show that the changes found within microglia inversely correlated with the density of dendritic spines on layer III pyramidal cells. These findings suggest microglia play a selective role in synaptic health within the aging brain

Lunar drill footplate and casing

To prevent hole collapse during lunar drilling operations, a casing has been devised of a graphite reinforced polyimide composite which will be able to withstand the lunar environment. Additionally, this casing will be inserted into the ground in segments two meters long which will penetrate the regolith simultaneously with the auger. The vertical action of the mobile platform will provide a downward force to the casing string through a special adaptor, giving the casing the needed impetus to sink the anticipated depth of ten meters. Casing segments will be connected with a simple snap arrangement. Excess casing will be cut off by a cylindrical cutting tool which will also transport the excess casing away from the hole. A footplate will be incorporated to grasp the auger rod string during rod segment additions or removals. The footplate grasping mechanism will consist of a set of vice-like arms, one end of each bearing threaded to a common power screw. The power screw will be threaded such that one end's thread pitch opposes that of the other end. The weight of the auger and rod string will be transmitted through the arms to the power screw and absorbed by a set of three ball bearing assemblies. The power screw will be driven by a one-half horsepower brushless motor actuated by radio control. The footplate will rest on four short legs and be anchored with pins that are an integral part of each leg

Differences between proposed Apollo sites: 1. Synthesis

Recent observations of the spectral reflectivity and emissivity of the five prime Apollo landing sites are evaluated in the context of similar observations of other localities on the moon and of data returned from unmanned lunar probes. We conclude that those five sites differ significantly only in minor constituents and/or relative valence states and that those differences are more modest than the differences that characterize mare regions generally. Recommendations of priorities for the five prime Apollo sites are made based on their uniqueness for sample return. Sampling of other lunar localities displaying anomalous emissivities and extreme color differences will be required to ascertain the full range of lithologies that constitute the lunar surface